Fabricating microarrays of functional proteins using affinity contact printing

Abstract

High-resolution arrays of antibodies can be prepared in a highly parallel manner by a combination of affinity purification and microcontact printing. Arrays with lateral dimensions of between 100 and 3 μm were prepared by using planar, affinity stamps that were patterned by using various soft lithographic techniques. The fluorescence microscopy image shown demonstrates the placement of anti-chicken and anti-goat antibodies on a glass substrate from a stamp.