Microfluidic selection of library elements

Abstract

We demonstrate that biological libraries can be screened using microfluidics. A bacteriophage library encoding dodecapeptides is passed through a microfluidic chip wherein antibodies are immobilized. Bacteriophages exhibiting affinity for the antibody are retained and eluted in a subsequent step. The high degree of control over flow and binding conditions during the screening enables dramatic reduction of the native library in favor of bacteriophages binding to the antibody target. © 2008 CBMS.