One-step immunoassay on capillary driven microfluidics

In this paper we report a novel concept and its implementation for performing a sandwich fluorescence surface immunoassay using only 2 μL of sample, 4 nL of detection antibodies (dAbs), 2 min of assay time and one pipetting step. In particular, we use an inkjet printer to deposit minute quantities of dAbs in microchannels which economizes reagent and enables quick reconstitution of dAbs for fast assays. © 2008CBMS.