Anna Fomitcheva Khartchenko, Iago Pereiro, et al.
MicroTAS 2020
Surface assays such as ELISA are pervasive in clinics and research and predominantly standardized in microtiter plates (MTP). MTPs provide many advantages but are often detrimental to surface assay efficiency due to inherent mass transport limitations. Microscale flows can overcome these and largely improve assay kinetics. However, the disruptive nature of microfluidics with existing labware and protocols has narrowed its transformative potential. We present WellProbe, a novel microfluidic concept compatible with MTPs. With it, we show that immunoassays become more sensitive at low concentrations (up to 9× signal improvement in 12× less time), richer in information with 3–4 different kinetic conditions, and can be used to estimate kinetic parameters, minimize washing steps and non-specific binding, and identify compromised results. We further multiplex single-well assays combining WellProbe's kinetic regions with tailored microarrays. Finally, we demonstrate our system in a context of immunoglobulin subclass evaluation, increasingly regarded as clinically relevant.
Anna Fomitcheva Khartchenko, Iago Pereiro, et al.
MicroTAS 2020
Martina Hitzbleck, Laetitia Avrain, et al.
Lab on a Chip
Emmanuel Delamarche, Yuksel Temiz, et al.
Angewandte Chemie - International Edition
Linda K. Sundberg, Gregory M. Wallraff, et al.
Proceedings of SPIE - The International Society for Optical Engineering 2010