Multiplexed convection-enhanced kinetics in microtiter plates
Iago Pereiro, Anna Fomitcheva Khartchenko, et al.
MicroTAS 2020
The microfluidic probe (MFP) facilitates performing local chemistry on biological substrates by confining nanoliter volumes of liquids. Using one particular implementation of the MFP, the hierarchical hydrodynamic flow confinement (hHFC), multiple liquids are simultaneously brought in contact with a substrate. Local chemical action and liquid shaping using the hHFC, is exploited to create cell patterns by locally lysing and removing cells. By utilizing the scanning ability of the MFP, user-defined patterns of cell monolayers are created. This protocol enables rapid, real-time and spatially controlled cell patterning, which can allow selective cell-cell and cell-matrix interaction studies.
Iago Pereiro, Anna Fomitcheva Khartchenko, et al.
MicroTAS 2020
Nuri Murat Arar, Pushpak Pati, et al.
IEEE T-BME
Aditya Kashyap, Julien F. Cors, et al.
MicroTAS 2016
Robert D. Lovchik, Fabio Bianco, et al.
Lab on a Chip